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Asymmetrically Positioned Flagellar Control Units Regulate Human Sperm Rotation.
Miller, Melissa R; Kenny, Samuel J; Mannowetz, Nadja; Mansell, Steven A; Wojcik, Michal; Mendoza, Sarah; Zucker, Robert S; Xu, Ke; Lishko, Polina V.
Afiliación
  • Miller MR; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Kenny SJ; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Mannowetz N; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Mansell SA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Wojcik M; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Mendoza S; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Zucker RS; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Xu K; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; Chan Zuckerberg Biohub, San Francisco, CA 94158, USA. Electronic address: xuk@berkeley.edu.
  • Lishko PV; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA. Electronic address: lishko@berkeley.edu.
Cell Rep ; 24(10): 2606-2613, 2018 09 04.
Article en En | MEDLINE | ID: mdl-30184496
ABSTRACT
Ion channels control sperm navigation within the female reproductive tract and, thus, are critical for their ability to find and fertilize an egg. The flagellar calcium channel CatSper controls sperm hyperactivated motility and is dependent on an alkaline cytoplasmic pH. The latter is accomplished by either proton transporters or, in human sperm, via the voltage-gated proton channel Hv1. To provide concerted regulation, ion channels and their regulatory proteins must be compartmentalized. Here, we describe flagellar regulatory nanodomains comprised of Hv1, CatSper, and its regulatory protein ABHD2. Super-resolution microscopy revealed that Hv1 is distributed asymmetrically within bilateral longitudinal lines and that inhibition of this channel leads to a decrease in sperm rotation along the long axis. We suggest that specific distribution of flagellar nanodomains provides a structural basis for the selective activation of CatSper and subsequent flagellar rotation. The latter, together with hyperactivated motility, enhances the fertility of sperm.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Motilidad Espermática / Espermatozoides / Canales de Calcio / Flagelos Límite: Humans / Male Idioma: En Revista: Cell Rep Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Motilidad Espermática / Espermatozoides / Canales de Calcio / Flagelos Límite: Humans / Male Idioma: En Revista: Cell Rep Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos