A genetic approach to promoter recognition during trans induction of viral gene expression.
Science
; 234(4772): 53-9, 1986 Oct 03.
Article
en En
| MEDLINE
| ID: mdl-3018926
ABSTRACT
Viral infection of mammalian cells entails the regulated induction of viral gene expression. The induction of many viral genes, including the herpes simplex virus gene encoding thymidine kinase (tk), depends on viral regulatory proteins that act in trans. Because recognition of the tk promoter by cellular transcription factors is well understood, its trans induction by viral regulatory proteins may serve as a useful model for the regulation of eukaryotic gene expression. A comprehensive set of mutations was therefore introduced into the chromosome of herpes simplex virus at the tk promoter to directly analyze the effects of promoter mutations on tk transcription. The promoter domains required for efficient tk expression under conditions of trans induction corresponded to those important for recognition by cellular transcription factors. Thus, trans induction of tk expression may be catalyzed initially by the interaction of viral regulatory proteins with cellular transcription factors.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Activación Viral
/
Regulación de la Expresión Génica
/
Regiones Promotoras Genéticas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Science
Año:
1986
Tipo del documento:
Article