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Three-photon imaging of mouse brain structure and function through the intact skull.
Wang, Tianyu; Ouzounov, Dimitre G; Wu, Chunyan; Horton, Nicholas G; Zhang, Bin; Wu, Cheng-Hsun; Zhang, Yanping; Schnitzer, Mark J; Xu, Chris.
Afiliación
  • Wang T; School of Applied and Engineering Physics, Cornell University, Ithaca, NY, USA. tw329@cornell.edu.
  • Ouzounov DG; School of Applied and Engineering Physics, Cornell University, Ithaca, NY, USA.
  • Wu C; School of Applied and Engineering Physics, Cornell University, Ithaca, NY, USA.
  • Horton NG; School of Applied and Engineering Physics, Cornell University, Ithaca, NY, USA.
  • Zhang B; CNC Program, Stanford University, Stanford, CA, USA.
  • Wu CH; CNC Program, Stanford University, Stanford, CA, USA.
  • Zhang Y; CNC Program, Stanford University, Stanford, CA, USA.
  • Schnitzer MJ; Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA.
  • Xu C; CNC Program, Stanford University, Stanford, CA, USA.
Nat Methods ; 15(10): 789-792, 2018 10.
Article en En | MEDLINE | ID: mdl-30202059
ABSTRACT
Optical imaging through the intact mouse skull is challenging because of skull-induced aberrations and scattering. We found that three-photon excitation provided improved optical sectioning compared with that obtained with two-photon excitation, even when we used the same excitation wavelength and imaging system. Here we demonstrate three-photon imaging of vasculature through the adult mouse skull at >500-µm depth, as well as GCaMP6s calcium imaging over weeks in cortical layers 2/3 and 4 in awake mice, with 8.5 frames per second and a field of view spanning hundreds of micrometers.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Cráneo / Procesamiento de Imagen Asistido por Computador / Encéfalo / Microscopía de Fluorescencia por Excitación Multifotónica / Neuroimagen Límite: Animals Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Cráneo / Procesamiento de Imagen Asistido por Computador / Encéfalo / Microscopía de Fluorescencia por Excitación Multifotónica / Neuroimagen Límite: Animals Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos