[Simultaneous determination of 12 rodenticides in whole blood and urine samples by high performance liquid chromatography-tandemmass spectrometry].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
; 36(7): 538-541, 2018 Jul 20.
Article
en Zh
| MEDLINE
| ID: mdl-30248773
ABSTRACT
Objective:
To develop a method for Simultaneous and rapid determination of 12 rodenticides including pindone, vacor, coumatetralyl, warfarin, diphacinone, coumachlor, chlorphacinon, difenacoum, brodifacoum, bromadiolone, difethialone and flocoumafen in whole blood and urine samples by high performance liquid chromatography-tandem mass (LC-MS-MS) .Methods:
The whole blood samples were precipitated with acetonitrile, purified by OstroTM 96-well plate, The urine samples were extracted by acetonitrile, and then separated on a ODS column, analyzed with high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) . The external standard calibration were tested.Results:
A good linearity was observed in their respective concentration ranges of 12 rodenticides. The related coefficients were 0.993 0~0.999 8. The limit of detections were 0.05 µg/L~1.4 µg/L. The rates of recovery were 92.5%~118.0%. The relative standard deviations were between 0.8%~17.3%.Conclusion:
The method was simple, rapid, sensitive, accurate and suitable for simultaneous detection of the 12 rodenticides in whole blood and urine samples of intoxicated patients.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Rodenticidas
/
Cromatografía Líquida de Alta Presión
/
Espectrometría de Masas en Tándem
Límite:
Humans
Idioma:
Zh
Revista:
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
Asunto de la revista:
MEDICINA OCUPACIONAL
Año:
2018
Tipo del documento:
Article
País de afiliación:
China