Culture and Molecular Profiling of the Respiratory Tract Microbiota.
Methods Mol Biol
; 1849: 49-61, 2018.
Article
en En
| MEDLINE
| ID: mdl-30298247
ABSTRACT
Microbiome research of host-associated communities has been advanced recently through improvements in sequencing technologies and bioinformatic methods. Traditional microbiological culture, when combined with molecular techniques, can provide a sensitive platform to comprehensively study the airway microbiota. Here we describe the culture methods necessary to capture a large proportion of the airway microbiota and molecular methods for profiling bacterial communities through the 16S rRNA gene, which, when combined, offer a more complete picture of the diversity of airway microbial communities than either method alone.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Sistema Respiratorio
/
Bacterias
/
ADN Bacteriano
/
ARN Ribosómico 16S
/
Análisis de Secuencia de ADN
/
Perfilación de la Expresión Génica
/
Microbiota
Límite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2018
Tipo del documento:
Article
País de afiliación:
Canadá