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[Regulatory effect of Shudihuang on expressions of BDNF/TrkB and NRG-3 in prefrontal cortex and striatum of ADHD model rats].
Yuan, Hai-Xia; Ni, Xin-Qiang; Wu, Zheng-Zhi; Han, Xin-Min; Xu, Dan-Gui; Song, Yu-Chen; Chen, Tian-Yi.
Afiliación
  • Yuan HX; Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing 210023, China.
  • Ni XQ; Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518038, China.
  • Wu ZZ; Shenzhen Institute of Geriatrics, Shenzhen 518035, China.
  • Han XM; Shenzhen Institute of Geriatrics, Shenzhen 518035, China.
  • Xu DG; Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing 210023, China.
  • Song YC; Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518038, China.
  • Chen TY; Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Zhongguo Zhong Yao Za Zhi ; 43(17): 3539-3544, 2018 Sep.
Article en Zh | MEDLINE | ID: mdl-30347924
ABSTRACT
To observe the effect of Shudihuang on behaviors and expression of BDNF/TrkB and NRG-3 in prefrontal cortex and striatum of attention deficit hyperactivity disorder (ADHD) model rats. Thirty 4-week-old spontaneous hypertension rats (SHR) were randomly divided into model group, methylphenidate hydrochloride (MPH, 2 mg·kg⁻¹·d⁻¹) and Shudihuang group (2.4 g·kg⁻¹·d⁻¹). Another 10 Wistar-Kyoto (WKY) rats were selected as normal control group. The 0.5% CMC-Na solution was administered to model group and WKY rats (2 mL·kg⁻¹·d⁻¹). All of the rats were treated for 4 weeks. The open field test was performed at the 14th and 28th days after gavage, in order to evaluate the spontaneous and impulsive behaviors. Subsequently, gene and protein expressions of BDNF/TrkB and NRG-3 were tested by RT-qPCR and Western blot. Compared with model group, MPH and Shudihuang groups showed significant reduction in total distance, mean velocity and central distance in the open field test (P<0.05), and Shudihuang group displayed a shorter central distance than MPH group (P<0.05). RT-qPCR and Western blot analysis indicated that expressions of BDNF/TrkB and NRG-3 were lower in prefrontal cortex and striatum of SHR compared with WKY rats. Four weeks later after administration, both Shudihuang and MPH significantly elevated mRNA and protein expressions of BDNF/TrkB and NRG-3 (P<0.05).In conclusion, neurodevelopmental disorder mediated by BDNF/TrkB and NRG-3 was closely related with SHR rats' behaviors. Shudihuang may ameliorate the spontaneous and impulsive behaviors by up-regulating the expressions of BDNF/TrkB and NRG-3 and improving growth and maturation of neurons in SHR.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Trastorno por Déficit de Atención con Hiperactividad / Medicamentos Herbarios Chinos / Corteza Prefrontal / Factor Neurotrófico Derivado del Encéfalo / Cuerpo Estriado / Receptor trkB / Neurregulinas / Péptidos y Proteínas de Señalización Intracelular Tipo de estudio: Prognostic_studies Límite: Animals Idioma: Zh Revista: Zhongguo Zhong Yao Za Zhi Asunto de la revista: FARMACOLOGIA / TERAPIAS COMPLEMENTARES Año: 2018 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Trastorno por Déficit de Atención con Hiperactividad / Medicamentos Herbarios Chinos / Corteza Prefrontal / Factor Neurotrófico Derivado del Encéfalo / Cuerpo Estriado / Receptor trkB / Neurregulinas / Péptidos y Proteínas de Señalización Intracelular Tipo de estudio: Prognostic_studies Límite: Animals Idioma: Zh Revista: Zhongguo Zhong Yao Za Zhi Asunto de la revista: FARMACOLOGIA / TERAPIAS COMPLEMENTARES Año: 2018 Tipo del documento: Article País de afiliación: China