Measuring Both Glucose Uptake and Myosin Heavy Chain Isoform Expression in Single Rat Skeletal Muscle Fibers.
Methods Mol Biol
; 1889: 283-300, 2019.
Article
en En
| MEDLINE
| ID: mdl-30367421
ABSTRACT
Glucose uptake by skeletal muscle is important for metabolic health. Because skeletal muscle is composed of multiple fiber types that have differing metabolic and contractile properties, studying glucose uptake in whole muscle tissue does not elucidate differences at the cellular level. Here, we describe a procedure that enables the measurement of both glucose uptake and fiber type (by myosin heavy chain isoform expression) in individual rat epitrochlearis muscle fibers.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fibras Musculares Esqueléticas
/
Cadenas Pesadas de Miosina
/
Glucosa
Límite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2019
Tipo del documento:
Article
País de afiliación:
Estados Unidos