T5 exonuclease-dependent assembly offers a low-cost method for efficient cloning and site-directed mutagenesis.

Xia, Yongzhen; Li, Kai; Li, Jingjing; Wang, Tianqi; Gu, Lichuan; Xun, Luying

Xia, Yongzhen; Li, Kai; Li, Jingjing; Wang, Tianqi; Gu, Lichuan; Xun, Luying.

Afiliación

Xia Y; State Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Road, Qingdao 266237, P.R. China.
Li K; State Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Road, Qingdao 266237, P.R. China.
Li J; State Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Road, Qingdao 266237, P.R. China.
Wang T; State Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Road, Qingdao 266237, P.R. China.
Gu L; State Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Road, Qingdao 266237, P.R. China.
Xun L; State Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Road, Qingdao 266237, P.R. China.

Nucleic Acids Res ; 47(3): e15, 2019 02 20.

Article en En | MEDLINE | ID: mdl-30462336

Asunto(s)

Clonación Molecular/métodos; Exodesoxirribonucleasas; Mutagénesis Sitio-Dirigida/métodos; Escherichia coli/genética; Vectores Genéticos; Polietilenglicoles; Trometamina

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Mutagénesis Sitio-Dirigida / Clonación Molecular / Exodesoxirribonucleasas Tipo de estudio: Health_economic_evaluation Idioma: En Revista: Nucleic Acids Res Año: 2019 Tipo del documento: Article

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