Role of HMGB1 in Post-traumatic Endoplasmic Reticulum Stress in Rat Lung Tissues.
Fa Yi Xue Za Zhi
; 34(4): 347-351, 2018 Aug.
Article
en En, Zh
| MEDLINE
| ID: mdl-30465396
ABSTRACT
OBJECTIVES:
To explore the role of high mobility group B1 ï¼HMGB1ï¼ protein in the post-traumatic endoplasmic reticulum stress ï¼ERSï¼ in rat lung tissues.METHODS:
The rat model of acute lung injury was established by crushing the hind limbs of rats with standard weight. The first experiment was to divide rats into postural control group and crush groups ï¼6 h, 18 h and 30 h after crushingï¼. The second experiment was to divide rats into postural control group, 18 h crush group, HMGB1 inhibitor sodium butyrate ï¼SBï¼ group and 18 h crush+SB group. The protein expression changes of HMGB1 and ERS- related proteins ï¼GRP78, caspase-12, CHOP and IRE1αï¼ in rat lung tissues were detected with Western blotting. Meanwhile, the pathological changes of rat lungs were observed by HE stain.RESULTS:
Compared with the postural control group, the expression levels of ERS-related proteins ï¼GRP78, caspase-12, CHOP and IRE1αï¼ and HMGB1 protein in rat lung tissues by crushing the hind limbs of rats were obviously increased. The protein levels reduced at 30 h after crushing but were still higher than those of postural control group and obvious pathological changes of acute lung injury were observed simultaneously in rats. Compared with the 18 h crush group, the expression levels of the ERS-related proteins and HMGB1 protein in rat lung tissues were attenuated in 18 h crush+SB group, and the pathological changes of rat lung injury began to alleviate.CONCLUSIONS:
HMGB1-ERS pathway activated by traumatic stress can lead to acute lung injury in rats.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteína HMGB1
/
Estrés del Retículo Endoplásmico
/
Pulmón
Límite:
Animals
Idioma:
En
/
Zh
Revista:
Fa Yi Xue Za Zhi
Asunto de la revista:
JURISPRUDENCIA
Año:
2018
Tipo del documento:
Article
País de afiliación:
China