Role of Chemokine Receptor CCR4 and Regulatory T Cells in Wound Healing of Diabetic Mice.
J Invest Dermatol
; 139(5): 1161-1170, 2019 05.
Article
en En
| MEDLINE
| ID: mdl-30465800
ABSTRACT
Wound healing is a well-coordinated process that involves inflammatory mediators and cellular responses; however, if any disturbances are present during this process, tissue repair is impaired. Chronic wounds are one of the serious long-term complications associated with diabetes mellitus. The chemokine receptor CCR4 and its respective ligands, CCL17 and CCL22, are involved in regulatory T cell recruitment and activation in inflamed skin; however, the role of regulatory T cells in wounds is still not clear. Our aim was to investigate the role of CCR4 and regulatory T cells in cutaneous wound healing in diabetic mice. Alloxan-induced diabetic wild- type mice (diabetic) developed wounds that were difficult to heal, differently from CCR4-/- diabetic mice (CCR4-/- diabetic), and also from anti-CCL17/22 or anti-CD25-injected diabetic mice that presented with accelerated wound healing and fewer regulatory T cells in the wound bed. Consequently, CCR4-/- diabetic mice also presented with alteration on T cells population in the wound and draining lymph nodes; on day 14, these mice also displayed an increase of collagen fiber deposition. Still, cytokine levels were decreased in the wounds of CCR4-/- diabetic mice on day 2. Our data suggest that the receptor CCR4 and regulatory T cells negatively affect wound healing in diabetic mice.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Cicatrización de Heridas
/
Diabetes Mellitus Experimental
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Diabetes Mellitus Tipo 1
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Quimiocina CCL17
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Quimiocina CCL22
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Receptores CCR4
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Invest Dermatol
Año:
2019
Tipo del documento:
Article
País de afiliación:
Brasil