Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells.
Opt Express
; 26(19): 25211-25225, 2018 Sep 17.
Article
en En
| MEDLINE
| ID: mdl-30469626
ABSTRACT
We present a new approach for combining holographic optical tweezers with confocal Raman spectroscopy. Multiple laser foci, generated using a liquid-crystal spatial light modulator, are individually used for both optical trapping and excitation of spontaneous Raman spectroscopy from trapped objects. Raman scattering from each laser focus is spatially filtered using reflective apertures on a digital micro-mirror device, which can be reconfigured with flexible patterns at video rate. We discuss operation of the instrument, and performance and viability considerations for biological measurements. We then demonstrate the capability of the instrument for fast, flexible, and interactive manipulation with molecular measurement of interacting live cell systems.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Espectrometría Raman
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Bacterias
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Células Dendríticas
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Holografía
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Linfocitos T
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Pinzas Ópticas
Idioma:
En
Revista:
Opt Express
Asunto de la revista:
OFTALMOLOGIA
Año:
2018
Tipo del documento:
Article