Diterpenoids from the Chinese liverwort Frullania hamatiloba and their Nrf2 inducing activities.
Phytochemistry
; 158: 77-85, 2019 Feb.
Article
en En
| MEDLINE
| ID: mdl-30476899
ABSTRACT
Six previously undescribed labdane diterpenoids, frullanians A-F, along with five known diterpenoids, were isolated from the Chinese liverwort Frullania hamatiloba Stephani. Their structures were determined using NMR data, electronic circular dichroism (ECD) calculations as well as the single crystal X-ray diffraction measurement. NAD(P)H QR (quinone reductase) assay demonstrated that frullanian D and four known compounds displayed antioxidant effect mediated via Nrf2 (Nuclear factor-erythroid 2-related factor 2) induction. Further investigation of the most bioactive frullanian D in MOVAS cells revealed that it ameliorated H2O2-induced oxidative insults without toxicity by increasing cell viability, attenuating morphological changes, and reducing intracellular ROS production. In addition, frullanian D promoted the nuclear translocation of Nrf2 and upregulated the expressions of antioxidant proteins NQO1 (NAD(P)H quinone oxidoreductase 1) and γ-GCS (γ-glutamyl cysteine synthetase). Docking analysis using MOE software further supported the activation of the Nrf2 pathway by frullanian D.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Frullania
/
Diterpenos
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Factor 2 Relacionado con NF-E2
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Antioxidantes
Límite:
Animals
Idioma:
En
Revista:
Phytochemistry
Año:
2019
Tipo del documento:
Article
País de afiliación:
China