Role of phage Ï1 in two strains of Salmonella Rissen, sensitive and resistant to phage Ï1.
BMC Microbiol
; 18(1): 208, 2018 12 07.
Article
en En
| MEDLINE
| ID: mdl-30526475
ABSTRACT
BACKGROUND:
The study describes the Salmonella Rissen phage Ï1 isolated from the Ï1-sensitive Salmonella Rissen strain RW. The same phage was then used to select the resistant strain RRÏ1+, which can harbour or not Ï1.RESULTS:
Following this approach, we found that Ï1, upon excision from RW cells with mitomycin, behaves as a temperate phage lyses host cells and generates phage particles; instead, upon spontaneous excision from RRÏ1+ cells, it does not generate phage particles; causes loss of phage resistance; switches the O-antigen from the smooth to the rough phenotype, and favors the transition of Salmonella Rissen from the planktonic to the biofilm growth. The RW and RRÏ1+ strains differ by 10 genes; of these, only two (phosphomannomutase_1 and phosphomannomutase_2; both involved in the mannose synthesis pathway) display significant differences at the expression levels. This result suggests that phage resistance is associated with these two genes.CONCLUSIONS:
Phage Ï1 displays the unusual property of behaving as template as well as lytic phage. This feature was used by the phage to modulate several phases of Salmonella Rissen lifestyle.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fagos de Salmonella
/
Salmonella enterica
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
BMC Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2018
Tipo del documento:
Article
País de afiliación:
Italia