Your browser doesn't support javascript.
loading
A Passive Microfluidic Device Based on Crossflow Filtration for Cell Separation Measurements: A Spectrophotometric Characterization.
Faustino, Vera; Catarino, Susana O; Pinho, Diana; Lima, Rui A; Minas, Graça.
Afiliación
  • Faustino V; Microelectromechanical Systems Research Unit (CMEMS-UMinho), University of Minho, Campus de Azurém, 4800-058 Guimarães, Portugal. id5778@alunos.uminho.pt.
  • Catarino SO; MEtRICs, Mechanical Engineering Department, University of Minho, 4800-058 Guimarães, Portugal. id5778@alunos.uminho.pt.
  • Pinho D; Microelectromechanical Systems Research Unit (CMEMS-UMinho), University of Minho, Campus de Azurém, 4800-058 Guimarães, Portugal. scatarino@dei.uminho.pt.
  • Lima RA; MEtRICs, Mechanical Engineering Department, University of Minho, 4800-058 Guimarães, Portugal. diana@ipb.pt.
  • Minas G; Research Centre in Digitalization and Intelligent Robotics (CeDRI), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal. diana@ipb.pt.
Biosensors (Basel) ; 8(4)2018 Dec 09.
Article en En | MEDLINE | ID: mdl-30544881
Microfluidic devices have been widely used as a valuable research tool for diagnostic applications. Particularly, they have been related to the successful detection of different diseases and conditions by assessing the mechanical properties of red blood cells (RBCs). Detecting deformability changes in the cells and being able to separate those cells may be a key factor in assuring the success of detection of some blood diseases with diagnostic devices. To detect and separate the chemically modified RBCs (mimicking disease-infected RBCs) from healthy RBCs, the present work proposes a microfluidic device comprising a sequence of pillars with different gaps and nine different outlets used to evaluate the efficiency of the device by measuring the optical absorption of the collected samples. This latter measurement technique was tested to distinguish between healthy RBCs and RBCs chemically modified with glutaraldehyde. The present study indicates that it was possible to detect a slight differences between the samples using an optical absorption spectrophotometric setup. Hence, the proposed microfluidic device has the potential to perform in one single step a partial passive separation of RBCs based on their deformability.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Separación Celular / Técnicas Analíticas Microfluídicas / Eritrocitos Tipo de estudio: Diagnostic_studies Límite: Female / Humans Idioma: En Revista: Biosensors (Basel) Año: 2018 Tipo del documento: Article País de afiliación: Portugal

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Separación Celular / Técnicas Analíticas Microfluídicas / Eritrocitos Tipo de estudio: Diagnostic_studies Límite: Female / Humans Idioma: En Revista: Biosensors (Basel) Año: 2018 Tipo del documento: Article País de afiliación: Portugal