Directed Evolution of Sulfonylurea Esterase and Characterization of a Variant with Improved Activity.

Liu, Bin; Peng, Qian; Sheng, Mengyao; Hu, Shishan; Qian, Meng; Fan, Ben; He, Jian

Liu, Bin; Peng, Qian; Sheng, Mengyao; Hu, Shishan; Qian, Meng; Fan, Ben; He, Jian.

Afiliación

Liu B; Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences , Nanjing Agricultural University , Nanjing 210095 , Jiangsu , People's Republic of China.
Peng Q; Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences , Nanjing Agricultural University , Nanjing 210095 , Jiangsu , People's Republic of China.
Sheng M; Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences , Nanjing Agricultural University , Nanjing 210095 , Jiangsu , People's Republic of China.
Hu S; Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences , Nanjing Agricultural University , Nanjing 210095 , Jiangsu , People's Republic of China.
Qian M; Laboratory Centre of Life Science, College of Life Sciences , Nanjing Agricultural University , Nanjing 210095 , Jiangsu , People's Republic of China.
Fan B; College of Forest Resources and Environment , Nanjing Forestry University , Nanjing , Jiangsu , People's Republic of China.
He J; Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences , Nanjing Agricultural University , Nanjing 210095 , Jiangsu , People's Republic of China.

J Agric Food Chem ; 67(3): 836-843, 2019 Jan 23.

Article en En | MEDLINE | ID: mdl-30585487

Asunto(s)

Proteínas Bacterianas/genética; Proteínas Bacterianas/metabolismo; Esterasas/genética; Esterasas/metabolismo; Methylocystaceae/enzimología; Compuestos de Sulfonilurea/metabolismo; Proteínas Bacterianas/química; Evolución Molecular Dirigida; Esterasas/química; Variación Genética; Herbicidas/química; Herbicidas/metabolismo; Cinética; Methylocystaceae/química; Methylocystaceae/genética; Pirimidinas/química; Pirimidinas/metabolismo; Compuestos de Sulfonilurea/química; Tiofenos/química; Tiofenos/metabolismo

Palabras clave

activity improvement; enzymatic characteristics; error-prone PCR; molecular modeling; sulfonylurea de-esterification esterase SulE

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Compuestos de Sulfonilurea / Proteínas Bacterianas / Methylocystaceae / Esterasas Tipo de estudio: Prognostic_studies Idioma: En Revista: J Agric Food Chem Año: 2019 Tipo del documento: Article

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