Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies.
Virol J
; 15(1): 192, 2018 12 27.
Article
en En
| MEDLINE
| ID: mdl-30587193
ABSTRACT
Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
/
Pruebas Serológicas
/
Pruebas de Neutralización
/
Tamizaje Masivo
/
Virus Zika
/
Infección por el Virus Zika
/
Anticuerpos Antivirales
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
/
Screening_studies
Límite:
Humans
País/Región como asunto:
Caribe
/
Martinica
Idioma:
En
Revista:
Virol J
Asunto de la revista:
VIROLOGIA
Año:
2018
Tipo del documento:
Article
País de afiliación:
Francia