Ascorbic acid diminishes bone morphogenetic protein 2-induced osteogenic differentiation of muscle precursor cells.
Muscle Nerve
; 59(4): 501-508, 2019 04.
Article
en En
| MEDLINE
| ID: mdl-30623463
ABSTRACT
INTRODUCTION:
Muscle precursor cells (MPC) are integral to the maintenance of skeletal muscle and have recently been implicated in playing a role in bone repair. The primary objective of this study was to understand better the role of oxidative stress during the osteogenic differentiation of MPCs.METHODS:
Muscle precursor cells were treated with various combinations of ascorbic acid (AA), bone morphogenetic protein (BMP)-2, and either a superoxide dismutase analog (4-hydroxy-TEMPO [TEMPOL]) or polyethyleneglycol-conjugated catalase. Muscle precursor cell proliferation and differentiation were determined, and alkaline phosphatase activity was measured as an index of osteogenic differentiation.RESULTS:
After treatment with 200 µM AA, superoxide was increased 1.5-fold, whereas AA in combination with 100 ng/ml BMP-2 did not increase alkaline phosphatase (ALP) activity. When cells were treated with TEMPOL in combination with 100 ng/ml BMP-2 and 200 µM AA, ALP activity significantly increased.DISCUSSION:
These data suggest that increasing oxidative stress with AA induces sublethal oxidative stress that prevents BMP-2-induced osteogenic differentiation of MPCs. Muscle Nerve 59501-508, 2019.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Osteogénesis
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Ácido Ascórbico
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Diferenciación Celular
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Músculo Esquelético
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Mioblastos
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Proteína Morfogenética Ósea 2
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Antioxidantes
Límite:
Animals
Idioma:
En
Revista:
Muscle Nerve
Año:
2019
Tipo del documento:
Article
País de afiliación:
Canadá