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Comparable Responses in Male and Female Mice to Cerulein-Induced Chronic Pancreatic Injury and Recovery.
Obafemi, Tolulope F; Yu, Peter; Li, Jing; Davis, Joy M; Liu, Ka; Cheng, Binglu; Zhao, Xiurong; Shen, Qiang; Younes, Mamoun; Ko, Tien C; Cao, Yanna.
Afiliación
  • Obafemi TF; Department of Surgery, UTHealth, 6431 Fannin Street, Houston, TX 77030, USA.
  • Yu P; Department of Surgery, UTHealth, 6431 Fannin Street, Houston, TX 77030, USA.
  • Li J; Department of Surgery, UTHealth, 6431 Fannin Street, Houston, TX 77030, USA.
  • Davis JM; Department of Clinical Laboratory Science, The Affiliated Hospital of Qingdao University, 19 Jiangsu Road, Qingdao, Shandong 266003, China.
  • Liu K; Department of Surgery, UTHealth, 6431 Fannin Street, Houston, TX 77030, USA.
  • Cheng B; Department of Surgery, UTHealth, 6431 Fannin Street, Houston, TX 77030, USA.
  • Zhao X; Department of Surgery, UTHealth, 6431 Fannin Street, Houston, TX 77030, USA.
  • Shen Q; Department of Neurology, UTHealth, 6431 Fannin Street, Houston, TX 77030, USA.
  • Younes M; Department of Clinical Cancer Prevention, Division of Division of Cancer Prevention and Population Sciences, The University of Texas MD Anderson Cancer Center, Houston, TX 77230, USA.
  • Ko TC; Department of Pathology & Laboratory Medicine, UTHealth, 6431 Fannin Street, Houston, TX 77030, USA.
  • Cao Y; Department of Surgery, UTHealth, 6431 Fannin Street, Houston, TX 77030, USA.
JOP ; 19(5): 236-243, 2018 Sep.
Article en En | MEDLINE | ID: mdl-30636940
OBJECTIVE: The cerulein-induced mouse pancreatitis model is a well-established, commonly used representation of human chronic pancreatitis pathology. Although studies report sex-dependent differences in human chronic pancreatitis, there are no studies in this model directly comparing sex response to pancreatic injury and recovery. Therefore, we designed a study to investigate whether sex- dependent differences in chronic pancreatitis injury and recovery exist in the cerulein-induced pancreatitis model. METHODS: Adult male and female C57BL/6 mice were administered cerulein (50 µg/kg, 5 hourly intraperitoneal injections/day, 3 days/week) for 4 weeks to induce chronic pancreatitis; control mice received normal saline injections. Pancreata and blood were harvested at 4 days (as injury group) or 4 weeks (as recovery group) after the last injection. Amylase secretion was measured from the serum. Acinar injury was scored on H&E sections. Fibrosis was assessed by Sirius Red and collagen immunofluorescence staining. RESULTS: Compared to time-matched controls, injury group displayed decreased body and pancreas weight, and increased acinar injury and fibrosis, with no significant differences between males and females. Recovery group demonstrated recovery of body weight, partial recovery of pancreas weight, reversal of acinar injury, and partial reversal of fibrosis, with no significant differences between males and females. Amylase secretion/body weight was similar across all groups. CONCLUSIONS: Male and female mice of the cerulein-induced chronic pancreatitis demonstrate similar responses to chronic pancreatitis injury and recovery. Although this model may not sufficiently emulate sex-dependent responses in human chronic pancreatitis, our study supports that both sexes of mice from this model can be used for the study of chronic pancreatitis.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: JOP Asunto de la revista: ENDOCRINOLOGIA / GASTROENTEROLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: JOP Asunto de la revista: ENDOCRINOLOGIA / GASTROENTEROLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos