Effect of Shenfu injection on lipopolysaccharide (LPS)-induced septic shock in rabbits.
J Ethnopharmacol
; 234: 36-43, 2019 Apr 24.
Article
en En
| MEDLINE
| ID: mdl-30641104
ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE Shenfu injection is a popular Chinese herbal formula that has been widely used in the treatment of shock in China. AIM OF THE STUDY To investigate the effect of Shenfu injection on lipopolysaccharide (LPS)-induced septic shock in rabbits. MATERIALS AND METHODS:
We established a septic shock model in rabbits by administering an intravenous injection of 0.6â¯mg/kg LPS to anesthetized rabbit, and 15â¯min after LPS challenge, the rabbits were intravenously administered the Shenfu injection. In these in vivo experiments, the jugular vein of the rabbits was cannulated for LPS and drug administration, and the right common carotid artery was cannulated to record the mean arterial pressure (MAP) over a 6-h period. In addition, various serum biochemical parameters, including lactate dehydrogenase (LDH), aspartate aminotransferase (AST), glutamate transaminase (ALT), creatinine (Cre), and urea nitrogen (Urea), were measured at 0, 3, and 6â¯h. Serum LPS levels at 6â¯h were determined by the test kit. And histological changes in the heart, liver and kidney tissues were observed by HE staining. Furthermore, some related small molecules in the heart tissues were detected by MALDI-TOF-MSI.RESULTS:
We found that Shenfu injection can increase the MAP, decrease the serum LPS, LDH and AST levels, and improve the tissue morphology of the heart, liver and kidney in rabbits with LPS-induced septic shock. In addition, Shenfu injection can increase the contents of ATP and taurine while reducing the content of AMP in the heart tissue during septic shock.CONCLUSIONS:
These results indicate that Shenfu injection exerts a protective effect on LPS-induced septic shock in rabbits.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Choque Séptico
/
Medicamentos Herbarios Chinos
/
Presión Arterial
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Ethnopharmacol
Año:
2019
Tipo del documento:
Article