Coprs inactivation leads to a derepression of LINE1 transposons in spermatocytes.
FEBS Open Bio
; 9(1): 159-168, 2019 01.
Article
en En
| MEDLINE
| ID: mdl-30652083
ABSTRACT
Repression of retrotransposons is essential for genome integrity during germ cell development and is tightly controlled through epigenetic mechanisms. In primordial germ cells, protein arginine N-methyltransferase (Prmt5) is involved in retrotransposon repression by methylating Piwi proteins, which is part of the piRNA pathway. Here, we show that in mice, genetic inactivation of coprs (which is highly expressed in testis and encodes a histone-binding protein required for the targeting of Prmt5 activity) affects the maturation of spermatogonia to spermatids. Mass spectrometry analysis revealed the presence of Miwi in testis protein lysates immunoprecipitated with an anti-Coprs antibody. The observed deregulation of Miwi and pachytene pre-piRNAs levels and the derepression of LINE1 repetitive sequences observed in coprs-/- mice suggest that Coprs is implicated in genome surveillance mechanisms.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Espermatocitos
/
Espermatogénesis
/
Elementos de Nucleótido Esparcido Largo
/
Chaperonas de Histonas
Límite:
Animals
Idioma:
En
Revista:
FEBS Open Bio
Año:
2019
Tipo del documento:
Article
País de afiliación:
Francia