Engineering the flagellar type III secretion system: improving capacity for secretion of recombinant protein.

Green, Charlotte A; Kamble, Nitin S; Court, Elizabeth K; Bryant, Owain J; Hicks, Matthew G; Lennon, Christopher; Fraser, Gillian M; Wright, Phillip C; Stafford, Graham P

Green, Charlotte A; Kamble, Nitin S; Court, Elizabeth K; Bryant, Owain J; Hicks, Matthew G; Lennon, Christopher; Fraser, Gillian M; Wright, Phillip C; Stafford, Graham P.

Afiliación

Green CA; Integrated BioSciences, School of Clinical Dentistry, University of Sheffield, Sheffield, S10 2TA, UK.
Kamble NS; Sustainable Process Technologies, Chemical and Environmental Engineering, University of Nottingham, Nottingham, NG7 2RD, UK.
Court EK; Integrated BioSciences, School of Clinical Dentistry, University of Sheffield, Sheffield, S10 2TA, UK.
Bryant OJ; Integrated BioSciences, School of Clinical Dentistry, University of Sheffield, Sheffield, S10 2TA, UK.
Hicks MG; Department of Pathology, University of Cambridge, Cambridge, CB2 1QP, UK.
Lennon C; Integrated BioSciences, School of Clinical Dentistry, University of Sheffield, Sheffield, S10 2TA, UK.
Fraser GM; FUJIFILM Diosynth Biotechnologies, Belasis Avenue, Stockton-on-Tees, Billingham, TS23 1LH, UK.
Wright PC; Department of Pathology, University of Cambridge, Cambridge, CB2 1QP, UK.
Stafford GP; School of Engineering, The Faculty of Science, Agriculture and Engineering, Newcastle University, Newcastle, NE1 7RU, UK.

Microb Cell Fact ; 18(1): 10, 2019 Jan 18.

Article en En | MEDLINE | ID: mdl-30657054

Asunto(s)

Escherichia coli/metabolismo; Ingeniería Metabólica; Sistemas de Secreción Tipo III/metabolismo; Regiones no Traducidas 5'; Hidrolasas de Éster Carboxílico/genética; Hidrolasas de Éster Carboxílico/metabolismo; Escherichia coli/crecimiento & desarrollo; Proteínas de Escherichia coli/genética; Proteínas de Escherichia coli/metabolismo; Flagelos/metabolismo; Flagelina/genética; Hormona de Crecimiento Humana/genética; Hormona de Crecimiento Humana/metabolismo; Humanos; Proteínas Recombinantes/biosíntesis; Proteínas Recombinantes/genética; Tiorredoxinas/genética; Tiorredoxinas/metabolismo

Palabras clave

Biotechnology; Flagellar; Recombinant protein secretion; Secretion assay; Strain engineering; Synthetic biology

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Escherichia coli / Ingeniería Metabólica / Sistemas de Secreción Tipo III Límite: Humans Idioma: En Revista: Microb Cell Fact Asunto de la revista: BIOTECNOLOGIA / MICROBIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Reino Unido

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