Analysis of Tissue-Resident Immune Cells from Mouse Skin and Lungs by Flow Cytometry.
Methods Mol Biol
; 1913: 217-222, 2019.
Article
en En
| MEDLINE
| ID: mdl-30666610
ABSTRACT
The heterogeneity and complexity of nonlymphoid tissues has become a major obstacle for the study of immune populations. For this reason, the generation of highly reproducible protocols that allow the analysis of immune cells in these tissues has become crucial for clinical and preclinical research. Here we describe an optimized method that allows the obtention of single-cell suspensions from the skin and lungs to analyze and quantify populations of tissue-resident memory CD8+ T cells by multi-parametric flow cytometry.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Piel
/
Separación Celular
/
Linfocitos T CD8-positivos
/
Citometría de Flujo
/
Pulmón
Tipo de estudio:
Guideline
Límite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2019
Tipo del documento:
Article
País de afiliación:
Chile