Imaging Macrophage Phagocytosis Using AIE Luminogen-Labeled E.â
coli.
Chem Asian J
; 14(6): 775-780, 2019 Mar 15.
Article
en En
| MEDLINE
| ID: mdl-30698362
ABSTRACT
Phagocytosis of bacteria is an important biological process. Gaining insight into this process may greatly benefit related pathological studies and further contribute to development of therapies for infectious diseases. Tools for studying these internalization processes, however, are limited. Herein, we demonstrate the feasibility of employing an environmentally sensitive aggregation-induced emission (AIE) probe for bacteria labeling and imaging. By tracking the fluorescence variation of the stained bacteria, the pH changes of its microenvironment can be monitored. In this way, the phagocytic entry of these bacteria into the macrophage cells and the intravacuolar acidification can be visualized in real-time.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Escherichia coli
/
Colorantes Fluorescentes
/
Macrófagos
Límite:
Animals
Idioma:
En
Revista:
Chem Asian J
Año:
2019
Tipo del documento:
Article
País de afiliación:
China