Establishment of Stable Cell Lines from Primary Human Dental Pulp Stem Cells.

Guirado, Elizabeth; Zhang, Youbin; George, Anne

Guirado, Elizabeth; Zhang, Youbin; George, Anne.

Afiliación

Guirado E; Department of Oral Biology, College of Dentistry, The University of Illinois at Chicago, Chicago, IL, USA.
Zhang Y; Department of Oral Biology, College of Dentistry, The University of Illinois at Chicago, Chicago, IL, USA.
George A; Department of Oral Biology, College of Dentistry, The University of Illinois at Chicago, Chicago, IL, USA. anneg@uic.edu.

Methods Mol Biol ; 1922: 21-27, 2019.

Article en En | MEDLINE | ID: mdl-30838561

ABSTRACT

ABSTRACT

This protocol is for the isolation of primary human dental pulp stem cells (DPSCs) from adult extracted molars and for the generation of high-titer lentivirus for in vitro infection of the DPSCs. Stable cell lines of dental pulp stem cells are generated, maintained in culture, and used for subsequent experiments.

Asunto(s)

Técnicas de Cultivo de Célula/métodos; Separación Celular/métodos; Pulpa Dental/citología; Diente Molar/citología; Células Madre; Adulto; Línea Celular; Técnicas de Transferencia de Gen; Humanos; Lentivirus

Palabras clave

Gene transfer techniques; Genetic recombination; Genetic transduction; Lentivirus; Somatic stem cells

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Células Madre / Separación Celular / Técnicas de Cultivo de Célula / Pulpa Dental / Diente Molar Límite: Adult / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos

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