[Alleles dropout patterns of Y-short tandem repeats in infertile males with Y chromosome microdeletions].
Yi Chuan
; 41(3): 243-253, 2019 Mar 20.
Article
en Zh
| MEDLINE
| ID: mdl-30872260
ABSTRACT
Y chromosomal short tandem repeat (Y-STR) typing is the most commonly used genetic technique in forensic studies. However, there may be a limit to the application of Y-STR in forensic science as Y-STR loci are subject to loss or variation caused by the higher chromosomal structures' spontaneous mutation rate. Located in the long arm of the Y chromosome, azoospermia factor (AZF) have been shown to participate in spermatogenesis and its deletion could cause infertility. However, little is known about the Y-STR dropout pattern in individuals with Y chromosome microdeletions. In this study, 85 infertile males with Y chromosome interstitial deletion were identified and special Y-STR allele dropout patterns were analyzed by employing a Y-STR Commercial Kit and a Y chromosome Deletion Kit. Results demonstrate that AZF a region deletion are related to DYS439-DYS389I-DYS389II alleles dropout, while AZF b region or c region deletions correlate to DYS448 allele dropout. Null DYS385-DYS392-DYS448 alleles were observed in AZF b+c+d region deletion individuals. While null DYS390-Y-GATA-H4-DYS385-DYS392-DYS448 alleles were observed in AZF a+b+c+d large region deletion individuals. Our data suggest that Y chromosome microdeletions may indicate specific Y-STR locus dropout patterns.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Repeticiones de Microsatélite
/
Alelos
/
Trastornos de los Cromosomas Sexuales del Desarrollo Sexual
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Infertilidad Masculina
Tipo de estudio:
Prognostic_studies
Límite:
Humans
/
Male
Idioma:
Zh
Revista:
Yi Chuan
Asunto de la revista:
GENETICA
Año:
2019
Tipo del documento:
Article
País de afiliación:
China