Monoclonal antibodies to the guanine-nucleotide binding proteins of adenylate cyclase.
Biochem J
; 236(1): 267-71, 1986 May 15.
Article
en En
| MEDLINE
| ID: mdl-3098233
ABSTRACT
Monoclonal antibodies (Mabs) to the stimulatory (Ns) and inhibitory (Ni) guanine nucleotide regulatory proteins associated with adenylate cyclase have been developed. Two Mabs (2A3 and 5G12), which are of the IgG2b subclass, recognize the beta-subunits (beta) of Ns, Ni and transducin. Iodinated beta can be immunoprecipitated by either Mab coupled to Affi-Gel 10 and this can be decreased by prior incubation of the Mabs with excess unlabelled beta. The Mabs stabilize the activated state of Ns while decreasing the rate of deactivation of activated Ns in the presence of beta.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Adenilil Ciclasas
/
Proteínas de Unión al GTP
/
Anticuerpos Monoclonales
Límite:
Animals
Idioma:
En
Revista:
Biochem J
Año:
1986
Tipo del documento:
Article