Haem iron reshapes colonic luminal environment: impact on mucosal homeostasis and microbiome through aldehyde formation.
Microbiome
; 7(1): 72, 2019 05 06.
Article
en En
| MEDLINE
| ID: mdl-31060614
ABSTRACT
BACKGROUND:
The World Health Organization classified processed and red meat consumption as "carcinogenic" and "probably carcinogenic", respectively, to humans. Haem iron from meat plays a role in the promotion of colorectal cancer in rodent models, in association with enhanced luminal lipoperoxidation and subsequent formation of aldehydes. Here, we investigated the short-term effects of this haem-induced lipoperoxidation on mucosal and luminal gut homeostasis including microbiome in F344 male rats fed with a haem-enriched diet (1.5 µmol/g) 14-21 days.RESULTS:
Changes in permeability, inflammation, and genotoxicity observed in the mucosal colonic barrier correlated with luminal haem and lipoperoxidation markers. Trapping of luminal haem-induced aldehydes normalised cellular genotoxicity, permeability, and ROS formation on a colon epithelial cell line. Addition of calcium carbonate (2%) to the haem-enriched diet allowed the luminal haem to be trapped in vivo and counteracted these haem-induced physiological traits. Similar covariations of faecal metabolites and bacterial taxa according to haem-induced lipoperoxidation were identified.CONCLUSIONS:
This integrated approach provides an overview of haem-induced modulations of the main actors in the colonic barrier. All alterations were closely linked to haem-induced lipoperoxidation, which is associated with red meat-induced colorectal cancer risk.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Colon
/
Aldehídos
/
Microbiota
/
Hemo
/
Mucosa Intestinal
/
Hierro
Límite:
Animals
Idioma:
En
Revista:
Microbiome
Año:
2019
Tipo del documento:
Article
País de afiliación:
Francia