Shedding and survival of an intracellular pathogenic Endozoicomonas-like organism infecting king scallop Pecten maximus.
Dis Aquat Organ
; 134(2): 167-173, 2019 May 23.
Article
en En
| MEDLINE
| ID: mdl-31120042
ABSTRACT
The Lyme Bay marine protected area (MPA) hosts a valuable population of king scallop Pecten maximus L. Recently, an Endozoicomonas-like organism (ELO), infecting host gill epithelial tissue, was associated with king scallop mass mortality events within the Lyme Bay MPA. Currently, very little is known about its transmission and survival outside the host. In this investigation, animals collected outside of reported mortality events showed high levels of ELO infection. Gill tissue disruption and the release of bacteria into the interlamellar space was seen histologically, suggesting shedding of ELO from host animals. To investigate pathogen survival outside the host, infected scallops were maintained in static water for a 24 h period, and then removed. Over the subsequent 8 d, water samples were collected and the quantity of ELO 16S rRNA transcript was measured by TaqManTM quantitative PCR (qPCR). The 16S rRNA transcript quantity was stable outside the host for 6 d before bacteria survival declined 2 logs (7.9 × 108 16S rRNA to 2.3 × 106 transcripts), suggesting that ELO can survive independently outside the host organism. The ELO-specific qPCR probe can therefore be used in future field studies of ELO prevalence within the environment and fauna of the Lyme Bay MPA.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Pecten
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Dis Aquat Organ
Asunto de la revista:
BIOLOGIA
/
MEDICINA VETERINARIA
/
MICROBIOLOGIA
Año:
2019
Tipo del documento:
Article
País de afiliación:
Reino Unido