Corallincola holothuriorum sp. nov., a facultative anaerobe isolated from sea cucumber intestine.

ABSTRACT

Strain C4T, isolated from sea cucumber intestine in Weihai, Shandong, PR China, is a novel Gram-stain-negative, facultatively anaerobic, amphitrichously flagellated, short rod that grows as creamy white bacterial colonies on plates. Optimal growth of the strain was observed at 28-30 °C, pH 6.5-7.0 and at a concentration of 3 % NaCl. The G+C content of the genomic DNA was 49.0 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain C4T is a member of the genus Corallincola and was most similar to Corallincola platygyrae JLT2006T. The major cellular fatty acids of strain C4T were C16  1ω7c/iso-C15  0 2-OH, C16  0 and C18  1ω7c. The sole respiratory quinone was Q-8. The predominant polar lipids in strain C4T were phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid. Based on morphology and physiological characteristics, strain C4T should be classified as a novel species in the genus Corallincola, for which Corallincolaholothuriorum is proposed. The type strain is C4T (=ATCC BAA-2611T=CICC 10839T).