Spatiotemporally Super-Resolved Volumetric Traction Force Microscopy.
Nano Lett
; 19(7): 4427-4434, 2019 07 10.
Article
en En
| MEDLINE
| ID: mdl-31199151
ABSTRACT
Quantification of mechanical forces is a major challenge across biomedical sciences. Yet such measurements are essential to understanding the role of biomechanics in cell regulation and function. Traction force microscopy remains the most broadly applied force probing technology but typically restricts itself to single-plane two-dimensional quantifications with limited spatiotemporal resolution. Here, we introduce an enhanced force measurement technique combining 3D super-resolution fluorescence structural illumination microscopy and traction force microscopy (3D-SIM-TFM) offering increased spatiotemporal resolution, opening-up unprecedented insights into physiological three-dimensional force production in living cells.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Simulación por Computador
/
Microscopía de Fuerza Atómica
Idioma:
En
Revista:
Nano Lett
Año:
2019
Tipo del documento:
Article
País de afiliación:
Reino Unido