Two-Photon Absorption and Cell Imaging of Fluorene-Functionalized Epicocconone Analogues.
Chemistry
; 25(46): 10954-10964, 2019 Aug 14.
Article
en En
| MEDLINE
| ID: mdl-31215691
ABSTRACT
Epicocconone 1 is a natural chromophore isolated from the fungus Epicoccum nigrum that has shown applications in proteomics and fluorescent microscopy thanks to its unique pro-fluorescence properties. The modification of the skeleton of the natural product by replacing the triene side chain by a fluorenyl scaffold can noticeably increase the fluorophore's absorption coefficient. The synthesis of the analogues of the natural product has been made possible by the use of a palladium-catalyzed carbonylation reaction, allowing the construction of the ß-keto-dioxinone key intermediate. Two-photon absorption cross-section measurements of the fluorenyl epicocconone analogues show a structure dependency with values ranging from 60 to 280â
GM and live cell imaging show intense staining of intracellular vesicle-like structures around the nucleus.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Benzopiranos
/
Microscopía de Fluorescencia por Excitación Multifotónica
/
Fluorenos
/
Colorantes Fluorescentes
/
Furanos
/
Cetonas
Límite:
Animals
Idioma:
En
Revista:
Chemistry
Asunto de la revista:
QUIMICA
Año:
2019
Tipo del documento:
Article
País de afiliación:
Francia