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A sensitive antibody-free 2D-LC-MS/MS assay for the quantitation of myostatin in the serum of different species.
Zhang, Guodong; Chen, Siwei; Song, Kening; Pan, Peng; Qiu, Yongchang; Amaravadi, Lakshmi; Wu, Jiang.
Afiliación
  • Zhang G; Bioanalytical & Biomarker Development, Takeda Pharmaceuticals International Co., 125 Binney Street, Cambridge, MA 02142, USA.
  • Chen S; Bioanalytical & Biomarker Development, Takeda Pharmaceuticals International Co., 125 Binney Street, Cambridge, MA 02142, USA.
  • Song K; Bioanalytical & Biomarker Development, Takeda Pharmaceuticals International Co., 125 Binney Street, Cambridge, MA 02142, USA.
  • Pan P; Bioanalytical & Biomarker Development, Takeda Pharmaceuticals International Co., 125 Binney Street, Cambridge, MA 02142, USA.
  • Qiu Y; Bioanalytical & Biomarker Development, Takeda Pharmaceuticals International Co., 125 Binney Street, Cambridge, MA 02142, USA.
  • Amaravadi L; Bioanalytical & Biomarker Development, Takeda Pharmaceuticals International Co., 125 Binney Street, Cambridge, MA 02142, USA.
  • Wu J; Bioanalytical & Biomarker Development, Takeda Pharmaceuticals International Co., 125 Binney Street, Cambridge, MA 02142, USA.
Bioanalysis ; 11(10): 957-970, 2019 May.
Article en En | MEDLINE | ID: mdl-31218899
ABSTRACT

Aim:

Myostatin (MSTN) is an attractive therapeutic target for the treatment of muscle degeneration-related diseases and is being evaluated as a target engagement biomarker.

Methods:

A sensitive 2D-LC-MS/MS assay was developed to quantify MSTN in different animal species. Sample preparation involved SDS denaturation of serum proteins followed by tryptic digestion and peptide enrichment by SPE.

Results:

The assay was validated with LLOQ of 2.5 ng/ml in rat and monkey serum. The precision was within 13.7%, and the bias was within ±12.6% for all quality control samples in authentic matrices.

Conclusion:

This new assay was successfully applied to measure MSTN in mouse, rat, monkey and human serum. The total MSTN in rat and monkey serum was elevated following administration of an MSTN inhibitor.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Análisis Químico de la Sangre / Cromatografía Liquida / Espectrometría de Masas en Tándem / Miostatina / Límite de Detección Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: En Revista: Bioanalysis Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
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Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Análisis Químico de la Sangre / Cromatografía Liquida / Espectrometría de Masas en Tándem / Miostatina / Límite de Detección Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: En Revista: Bioanalysis Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos