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A novel simplified method of generating cytomegalovirus-specific cytokine-induced killer cells of high specificity and superior potency with GMP compliance.
Luah, Yen Hoon; Sundar Raj, Kirubavathy; Koh, Mickey B C; Linn, Yeh Ching.
Afiliación
  • Luah YH; Cell Therapy Facility, Health Sciences Authority, 11 Outram Rd, 169078, Singapore.
  • Sundar Raj K; Dept of Hematology, Singapore General Hospital, Outram Rd, 169608, Singapore.
  • Koh MBC; Cell Therapy Facility, Health Sciences Authority, 11 Outram Rd, 169078, Singapore.
  • Linn YC; Dept of Hematology, Singapore General Hospital, Outram Rd, 169608, Singapore. Electronic address: linn.yeh.ching@singhealth.com.sg.
Clin Immunol ; 205: 83-92, 2019 08.
Article en En | MEDLINE | ID: mdl-31229666
We describe a method of rendering polyclonal cytokine-induced killer cells (CIK) specific against cytomegalovirus (CMV), focusing on GMP compliance. Peripheral blood mononuclear cells (PBMNC) are stimulated with pooled CMV peptides pp65 and IE-1 for 16-24 h and the reactive T cell subset which up-regulate CD137 is further co-stimulated with anti-CD137, followed by expansion in G-Rex flasks under standard CIK culture condition. This method generates a large number CMV-specific CIK with superior potency compared to published method currently in clinical trials. The cytotoxicity as measured by chromium release assay correlates with the upregulation of CD107a upon peptide re-challenge as measured by flow cytometry. CMV-CIK at maturity consist of mainly late effector memory CD8 T cells and have a skewed TCR repertoire with preferential expansion of a few families. Such CMV-CIK retain their function after freezing and thawing. CMV-CIK thus generated is ready for clinical trial against drug-resistant CMV disease.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Linfocitos T Citotóxicos / Proteínas de la Matriz Viral / Subgrupos de Linfocitos T / Proteínas Inmediatas-Precoces / Técnicas de Cultivo de Célula / Citomegalovirus / Memoria Inmunológica Límite: Humans Idioma: En Revista: Clin Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Singapur

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Linfocitos T Citotóxicos / Proteínas de la Matriz Viral / Subgrupos de Linfocitos T / Proteínas Inmediatas-Precoces / Técnicas de Cultivo de Célula / Citomegalovirus / Memoria Inmunológica Límite: Humans Idioma: En Revista: Clin Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Singapur