DNAJC2 is required for mouse early embryonic development.
Biochem Biophys Res Commun
; 516(1): 258-263, 2019 08 13.
Article
en En
| MEDLINE
| ID: mdl-31230751
ABSTRACT
DNAJC2 protein, also known as ZRF1 or MPP11, acts both as chaperone and as chromatin regulator. It is involved in stem cell differentiation and its expression is associated with various cancer malignancies. However, the role of Dnajc2 gene during mouse embryogenesis has not been assessed so far. To this aim, we invalidated Dnajc2 gene in FVB/Nj mice using the CrispR/Cas9 approach. We showed that this invalidation leads to the early post-implantation lethality of the nullizygous embryos. Furthermore, using siRNAs against Dnajc2 in mouse 1-cell embryos, we showed that maternal Dnajc2 mRNAs may allow for the early preimplantation development of these embryos. Altogether, these data demonstrate for the first time the requirement of DNAJC2 for early mouse embryogenesis.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas de Unión al ARN
/
Chaperonas Moleculares
/
Regulación del Desarrollo de la Expresión Génica
/
Proteínas de Unión al ADN
/
Embrión de Mamíferos
/
Ratones
Límite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2019
Tipo del documento:
Article
País de afiliación:
Francia