Processing of high-salt-containing protein A eluate using mixed-mode chromatography in purifying an aggregation-prone antibody.

Zhang, Yuan; Cai, Lingli; Wang, Ying; Li, Yifeng

Zhang, Yuan; Cai, Lingli; Wang, Ying; Li, Yifeng.

Afiliación

Zhang Y; Technology and Process Development (TPD), WuXi Biologics, 288 Fute Zhong Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China.
Cai L; Technology and Process Development (TPD), WuXi Biologics, 288 Fute Zhong Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China.
Wang Y; Technology and Process Development (TPD), WuXi Biologics, 288 Fute Zhong Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China.
Li Y; Technology and Process Development (TPD), WuXi Biologics, 288 Fute Zhong Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China. Electronic address: li_yifeng@wuxiapptec.com.

Protein Expr Purif ; 164: 105458, 2019 12.

Article en En | MEDLINE | ID: mdl-31344473

Asunto(s)

Anticuerpos Monoclonales/aislamiento & purificación; Cloruros/química; Cromatografía por Intercambio Iónico/métodos; Proteína Estafilocócica A/química; Anticuerpos Monoclonales/química; Durapatita/química; Polietilenglicoles/química; Agregado de Proteínas

Palabras clave

Antibody aggregate; Capto MMC; Ceramic hydroxyapatite (CHT); Mixed-mode chromatography; Polyethylene glycol (PEG); Protein A chromatography

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteína Estafilocócica A / Cloruros / Cromatografía por Intercambio Iónico / Anticuerpos Monoclonales Idioma: En Revista: Protein Expr Purif Asunto de la revista: BIOLOGIA MOLECULAR Año: 2019 Tipo del documento: Article País de afiliación: China

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