The carbonic anhydrase of Clostridium autoethanogenum represents a new subclass of ß-carbonic anhydrases.
Appl Microbiol Biotechnol
; 103(17): 7275-7286, 2019 Sep.
Article
en En
| MEDLINE
| ID: mdl-31346685
ABSTRACT
Carbonic anhydrase catalyses the interconversion of carbon dioxide and water to bicarbonate and protons. It was unknown if the industrial-relevant acetogen Clostridium autoethanogenum possesses these enzymes. We identified two putative carbonic anhydrase genes in its genome, one of the ß class and one of the γ class. Carbonic anhydrase activity was found for the purified ß class enzyme, but not the γ class candidate. Functional complementation of an Escherichia coli carbonic anhydrase knock-out mutant showed that the ß class carbonic anhydrase could complement this activity, but not the γ class candidate gene. Phylogenetic analysis showed that the ß class carbonic anhydrase of Clostridium autoethanogenum represents a novel sub-class of ß class carbonic anhydrases that form the F-clade. The members of this clade have the shortest primary structure of any known carbonic anhydrase.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Anhidrasas Carbónicas
/
Clostridium
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Año:
2019
Tipo del documento:
Article
País de afiliación:
Reino Unido