Counting the Cuts: MAZTER-Seq Quantifies m6A Levels Using a Methylation-Sensitive Ribonuclease.

Garcia-Campos et al. describe MAZTER-seq, which deploys a sequence-specific, methylation-sensitive bacterial single-stranded ribonuclease MazF to provide nucleotide-resolution quantification of m6A methylation sites. The study reveals many new sites and supports the idea of a predictable "m6A code," where methylation levels are dictated primarily by local sequence at the site of methylation.