Gene manipulation in liver ductal organoids by optimized recombinant adeno-associated virus vectors.

Wei, Jinsong; Ran, Gai; Wang, Xin; Jiang, Ning; Liang, Jianqing; Lin, Xinhua; Ling, Chen; Zhao, Bing

Wei, Jinsong; Ran, Gai; Wang, Xin; Jiang, Ning; Liang, Jianqing; Lin, Xinhua; Ling, Chen; Zhao, Bing.

Afiliación

Wei J; State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai 200438, China.
Ran G; State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai 200438, China.
Wang X; State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai 200438, China.
Jiang N; State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai 200438, China.
Liang J; State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai 200438, China.
Lin X; State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai 200438, China xlin@fudan.edu.cn.
Ling C; Perinatal Institute, Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio 45229.
Zhao B; State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai 200438, China lingchenchina@fudan.edu.cn.

J Biol Chem ; 294(38): 14096-14104, 2019 09 20.

Article en En | MEDLINE | ID: mdl-31366731

Asunto(s)

Diferenciación Celular/genética; Ingeniería Genética/métodos; Hepatocitos/citología; Animales; Linaje de la Célula; Dependovirus/genética; Regulación de la Expresión Génica/genética; Conducto Hepático Común/citología; Factor Nuclear 4 del Hepatocito/genética; Factor Nuclear 4 del Hepatocito/metabolismo; Humanos; Hígado/citología; Ratones; Ratones Endogámicos C57BL; Organoides/citología; Transducción de Señal; Factores de Transcripción

Palabras clave

adeno-associated virus (AAV) vector; cholangiocyte-to-hepatocyte differentiation; differentiation; functional screening; gene manipulation; gene therapy; hepatocyte; liver; liver ductal organoid; signaling

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ingeniería Genética / Diferenciación Celular / Hepatocitos Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: J Biol Chem Año: 2019 Tipo del documento: Article País de afiliación: China

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