Single cell analysis of nutrient regulation of Clostridioides (Clostridium) difficile motility.
Anaerobe
; 59: 205-211, 2019 Oct.
Article
en En
| MEDLINE
| ID: mdl-31386902
ABSTRACT
Regulation of bacterial motility to maximize nutrient acquisition or minimize exposure to harmful substances plays an important role in microbial proliferation and host colonization. The technical difficulties of performing high-resolution live microscopy on anaerobes have hindered mechanistic studies of motility in Clostridioides (formerly Clostridium) difficile. Here, we present a widely applicable protocol for live cell imaging of anaerobic bacteria that has allowed us to characterize C. difficile swimming at the single-cell level. This accessible method for anaerobic live cell microscopy enables inquiry into previously inaccessible aspects of C. difficile physiology and behavior. We present the first report that vegetative C. difficile are capable of regulated motility in the presence of different nutrients. We demonstrate that the epidemic C. difficile strain R20291 exhibits regulated motility in the presence of multiple nutrient sources by modulating its swimming velocity. This is a powerful illustration of the ability of single-cell studies to explain population-wide phenomena such as dispersal through the environment.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Nutrientes
/
Clostridioides difficile
/
Microscopía Intravital
/
Locomoción
Idioma:
En
Revista:
Anaerobe
Año:
2019
Tipo del documento:
Article
País de afiliación:
Estados Unidos