Synergistic effects between the additions of a disulphide bridge and an N-terminal hydrophobic sidechain on the binding pocket tilting and enhanced Xyn11A activity.

Boonyaputthikul, Harit; Muhammad, Auwal; Roekring, Songsak; Rattanarojpong, Triwit; Khunrae, Pongsak; Sutthibutpong, Thana

Boonyaputthikul, Harit; Muhammad, Auwal; Roekring, Songsak; Rattanarojpong, Triwit; Khunrae, Pongsak; Sutthibutpong, Thana.

Afiliación

Boonyaputthikul H; Department of Microbiology, Science Laboratory Building, Faculty of Science, King Mongkut's University of Technology, Thonburi, Thailand.
Muhammad A; Theoretical and Computational Physics Group, Department of Physics, KMUTT, Thailand. Faculty of Science, King Mongkut's University of Technology, Thonburi, Thailand; Theoretical and Computational Science Center (TaCS), Science Laboratory Building, Faculty of Science, King Mongkut's University of Tec
Roekring S; Biochemical Department, Mitr-phol Biofuel, Ploenchit Center Building, Sukhumvit Road, Klongtoey, Bangkok, 10110, Thailand.
Rattanarojpong T; Department of Microbiology, Science Laboratory Building, Faculty of Science, King Mongkut's University of Technology, Thonburi, Thailand.
Khunrae P; Department of Microbiology, Science Laboratory Building, Faculty of Science, King Mongkut's University of Technology, Thonburi, Thailand. Electronic address: pongsak.khu@kmutt.ac.th.
Sutthibutpong T; Theoretical and Computational Physics Group, Department of Physics, KMUTT, Thailand. Faculty of Science, King Mongkut's University of Technology, Thonburi, Thailand; Theoretical and Computational Science Center (TaCS), Science Laboratory Building, Faculty of Science, King Mongkut's University of Tec

Arch Biochem Biophys ; 672: 108068, 2019 09 15.

Article en En | MEDLINE | ID: mdl-31401092

Asunto(s)

Proteínas Bacterianas/química; Disulfuros/química; Endo-1,4-beta Xilanasas/química; Bacillus firmus/enzimología; Proteínas Bacterianas/genética; Sitios de Unión; Biocatálisis; Cisteína/química; Endo-1,4-beta Xilanasas/genética; Pruebas de Enzimas; Escherichia coli/genética; Interacciones Hidrofóbicas e Hidrofílicas; Simulación de Dinámica Molecular; Mutagénesis Sitio-Dirigida; Mutación; Conformación Proteica

Palabras clave

GH11 xylanase; Molecular dynamics; Protein engineering; Synergism

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Endo-1,4-beta Xilanasas / Disulfuros Idioma: En Revista: Arch Biochem Biophys Año: 2019 Tipo del documento: Article País de afiliación: Tailandia

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