Evidence that a GTP binding protein regulates phosphorylation of the CD3 antigen in human T lymphocytes.
Biochem Biophys Res Commun
; 156(1): 328-34, 1988 Oct 14.
Article
en En
| MEDLINE
| ID: mdl-3140812
ABSTRACT
The role of guanine nucleotide binding regulatory proteins (G proteins) in the regulation of phosphorylation of the gamma subunit of the CD3 antigen has been examined. CD3 gamma chain phosphorylation in isolated T cell microsomes was stimulated by the G protein activator guanosine 5'-0 thiotriphosphate (GTP gamma S), but cyclic adenosine monophosphate and guanosine 5'-diphosphate were ineffective at inducing gamma chain phosphorylation. The effect of GTP gamma S was rapid and transient; a half maximal effect was observed with 50 microM of the nucleotide. gamma polypeptide phosphorylated in vitro in GTP gamma S stimulated microsomes incorporated phosphate on Serines 123 and 126. These data are consistent with the involvement of a G protein in the signalling mechanisms that regulate the phosphorylation of the CD3 gamma chain.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Linfocitos T
/
Antígenos de Diferenciación de Linfocitos T
/
Proteínas de Unión al GTP
Límite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1988
Tipo del documento:
Article
País de afiliación:
Reino Unido