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Synthetic Biology Tools for the Fast-Growing Marine Bacterium Vibrio natriegens.
Tschirhart, Tanya; Shukla, Vrinda; Kelly, Erin E; Schultzhaus, Zachary; NewRingeisen, Erin; Erickson, Jeffrey S; Wang, Zheng; Garcia, Whitney; Curl, Emaleigh; Egbert, Robert G; Yeung, Enoch; Vora, Gary J.
Afiliación
  • Tschirhart T; American Society for Engineering Education, Postdoctoral Fellowship Program , US Naval Research Laboratory , Washington , DC 20375 , United States of America.
  • Shukla V; Center for Bio/Molecular Science and Engineering , US Naval Research Laboratory , Washington , DC 20375 , United States of America.
  • Kelly EE; National Research Council, Postdoctoral Fellowship Program , US Naval Research Laboratory , Washington , DC 20375 , United States of America.
  • Schultzhaus Z; National Research Council, Postdoctoral Fellowship Program , US Naval Research Laboratory , Washington , DC 20375 , United States of America.
  • NewRingeisen E; Center for Bio/Molecular Science and Engineering , US Naval Research Laboratory , Washington , DC 20375 , United States of America.
  • Erickson JS; Center for Bio/Molecular Science and Engineering , US Naval Research Laboratory , Washington , DC 20375 , United States of America.
  • Wang Z; Center for Bio/Molecular Science and Engineering , US Naval Research Laboratory , Washington , DC 20375 , United States of America.
  • Garcia W; Earth and Biological Sciences Directorate , Pacific Northwest National Laboratory , Richland , Washington 99352 , United States of America.
  • Curl E; Biomolecular Science and Engineering , University of California Santa Barbara , Santa Barbara , California 93106 , United States of America.
  • Egbert RG; Biological Sciences Division , Pacific Northwest National Laboratory , Richland , Washington 99352 , United States of America.
  • Yeung E; Department of Mechanical Engineering , University of California Santa Barbara , Santa Barbara , California 93106 , United States of America.
  • Vora GJ; Center for Bio/Molecular Science and Engineering , US Naval Research Laboratory , Washington , DC 20375 , United States of America.
ACS Synth Biol ; 8(9): 2069-2079, 2019 09 20.
Article en En | MEDLINE | ID: mdl-31419124
ABSTRACT
The fast-growing nonmodel marine bacterium Vibrio natriegens has recently garnered attention as a host for molecular biology and biotechnology applications. In order to further its capabilities as a synthetic biology chassis, we have characterized a wide range of genetic parts and tools for use in V. natriegens. These parts include many commonly used resistance markers, promoters, ribosomal binding sites, reporters, terminators, degradation tags, origin of replication sequences, and plasmid backbones. We have characterized the behavior of these parts in different combinations and have compared their functionality in V. natriegens and Escherichia coli. Plasmid stability over time, plasmid copy numbers, and production load on the cells were also evaluated. Additionally, we tested constructs for chemical and optogenetic induction and characterized basic engineered circuit behavior in V. natriegens. The results indicate that, while most parts and constructs work similarly in the two organisms, some deviate significantly. Overall, these results will serve as a primer for anyone interested in engineering V. natriegens and will aid in developing more robust synthetic biology principles and approaches for this nonmodel chassis.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Vibrio / Biología Sintética Idioma: En Revista: ACS Synth Biol Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Vibrio / Biología Sintética Idioma: En Revista: ACS Synth Biol Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos