Direct culture-independent sequence typing of Taylorella equigenitalis obtained from genital swabs and frozen semen samples from South African horses.
J Vet Diagn Invest
; 31(5): 792-794, 2019 Sep.
Article
en En
| MEDLINE
| ID: mdl-31423914
ABSTRACT
We report herein the use of crude extracts obtained from samples of Taylorella equigenitalis-infected horses for the purpose of multi-locus sequence typing (MLST). Samples (n = 36) were collected from horses in South Africa from 1996 to 2017 34 from genital swabs (stored at -20°C for 2-3 y) and 2 from cryopreserved raw semen aliquots (stored at -70°C for 18 y) prior to assay. The MLST assay showed a single sequence type (ST), designated ST4, that supported a point introduction and thus a common source for the South African outbreak of contagious equine metritis.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Semen
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Infecciones por Bacterias Gramnegativas
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Taylorella equigenitalis
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Tipificación de Secuencias Multilocus
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Infecciones del Sistema Genital
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Enfermedades de los Caballos
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
País/Región como asunto:
Africa
Idioma:
En
Revista:
J Vet Diagn Invest
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2019
Tipo del documento:
Article