Long-term culture of human lung adenocarcinoma A549 cells enhances the replication of human influenza A viruses.
J Gen Virol
; 100(10): 1345-1349, 2019 10.
Article
en En
| MEDLINE
| ID: mdl-31424377
ABSTRACT
Long-term culture of the human lung adenocarcinoma cell line A549 promotes the differentiation of these cells toward an alveolar type II cell phenotype. Here, we evaluated the susceptibility of long-term cultured A549 cells to human influenza viruses. A549 cells were cultured continuously for 25 days (D25-A549) or 1 day (D1-A549) in Ham's F12K medium. Six human influenza A viruses grew much faster in D25-A549 cells than in D1-A549 cells; however, two influenza B viruses replicated poorly in both cell types. Two avian influenza viruses replicated efficiently in both cell types, with similar titres. Expression levels of human virus receptors were higher in D25-A549 cells than in D1-A549 cells. D25-A549 cells thus more efficiently support the replication of human influenza A viruses compared with D1-A549 cells. Our data suggest that long-term cultured A549 cells will be useful for influenza A virus research.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Virus de la Influenza A
/
Virus de la Influenza B
/
Replicación Viral
Límite:
Humans
Idioma:
En
Revista:
J Gen Virol
Año:
2019
Tipo del documento:
Article
País de afiliación:
Japón