Spatiotemporal Expression of Anticoagulation Factor Antistasin in Freshwater Leeches.
Int J Mol Sci
; 20(16)2019 Aug 16.
Article
en En
| MEDLINE
| ID: mdl-31426335
ABSTRACT
Antistasin, which was originally discovered in the salivary glands of the Mexican leech Haementeria officinalis, was newly isolated from Helobdella austinensis. To confirm the temporal expression of antistasin during embryogenesis, we carried out semi-quantitative RT-PCR. Hau-antistasin1 was uniquely expressed at stage 4 of the cleavage and was strongly expressed in the late stages of organogenesis, as were other antistasin members. In order to confirm the spatial expression of antistasin, we performed fluorescence in situ hybridization in the late stages of organogenesis. The expression of each antistasin in the proboscis showed a similar pattern and varied in expression in the body. In addition, the spatial expression of antistasin orthologs in different leeches showed the possibility of different function across leech species. Hau-antistasin1 was expressed in the same region as hedgehog, which is a known mediator of signal transduction pathway. Hau-antistasin1 is probably a downstream target of Hedgehog signaling, involved in segment polarity signal pathway.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Hormonas de Invertebrados
/
Sanguijuelas
/
Anticoagulantes
Límite:
Animals
Idioma:
En
Revista:
Int J Mol Sci
Año:
2019
Tipo del documento:
Article