Metabolomic stability of exercise-induced sweat.
J Chromatogr B Analyt Technol Biomed Life Sci
; 1126-1127: 121763, 2019 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-31430684
ABSTRACT
Due to increased interest in the use of excreted sweat for biomarker discovery, data must be generated supporting sample collection and handling methods to allow for controlled, large-scale biomarker discovery studies to be performed. In this manuscript, twelve amino acids were quantitated from exercise-induced excreted sweat held at room temperature or a simulated body temperature of 37⯰C for up to 90â¯min. The data illustrate a large dynamic range exists among amino acids in sweat. Additionally, the amino acid quantities vary across individuals and among the same individual under different storage conditions, with alanine, arginine, and threonine showing a significant statistical difference between sampling events (pâ¯<â¯0.05). Furthermore, the results establish amino acids are relatively invariant, at both storage temperatures tested, for up to 90â¯min illustrated by <10% (15/156) of the amino acids measurements demonstrating change greater than 10% from the time zero value. An untargeted metabolomics approach was also applied to the data set to evaluate global changes to the metabolome. The results show more than 88% of all data points fall within the established limits, regardless of temperature condition and ionization mode. Collectively, this study demonstrates that sweat is largely invariant at two distinct temperatures for up to 90â¯min. These results establish sweat collection and sample handling is possible for up to 90â¯min with minimal changes in metabolite abundances.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Sudor
/
Metaboloma
/
Metabolómica
Límite:
Humans
/
Male
Idioma:
En
Revista:
J Chromatogr B Analyt Technol Biomed Life Sci
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2019
Tipo del documento:
Article