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Dual-chambered membrane bioreactor for coculture of stratified cell populations.
Navarro, Javier; Swayambunathan, Jay; Janes, Morgan Elizabeth; Santoro, Marco; Mikos, Antonios G; Fisher, John P.
Afiliación
  • Navarro J; Fischell Department of Bioengineering, University of Maryland, College Park, Maryland.
  • Swayambunathan J; Center for Engineering Complex Tissues, University of Maryland, College Park, Maryland.
  • Janes ME; Fischell Department of Bioengineering, University of Maryland, College Park, Maryland.
  • Santoro M; Center for Engineering Complex Tissues, University of Maryland, College Park, Maryland.
  • Mikos AG; Fischell Department of Bioengineering, University of Maryland, College Park, Maryland.
  • Fisher JP; Center for Engineering Complex Tissues, University of Maryland, College Park, Maryland.
Biotechnol Bioeng ; 116(12): 3253-3268, 2019 12.
Article en En | MEDLINE | ID: mdl-31502660
ABSTRACT
We have developed a dual-chambered bioreactor (DCB) that incorporates a membrane to study stratified 3D cell populations for skin tissue engineering. The DCB provides adjacent flow lines within a common chamber; the inclusion of the membrane regulates flow layering or mixing, which can be exploited to produce layers or gradients of cell populations in the scaffolds. Computational modeling and experimental assays were used to study the transport phenomena within the bioreactor. Molecular transport across the membrane was defined by a balance of convection and diffusion; the symmetry of the system was proven by its bulk convection stability, while the movement of molecules from one flow line to the other is governed by coupled convection-diffusion. This balance allowed the perfusion of two different fluids, with the membrane defining the mixing degree between the two. The bioreactor sustained two adjacent cell populations for 28 days, and was used to induce indirect adipogenic differentiation of mesenchymal stem cells due to molecular cross-talk between the populations. We successfully developed a platform that can study the dermis-hypodermis complex to address limitations in skin tissue engineering. Furthermore, the DCB can be used for other multilayered tissues or the study of communication pathways between cell populations.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Diferenciación Celular / Técnicas de Cultivo de Célula / Reactores Biológicos / Adipogénesis / Células Madre Mesenquimatosas / Membranas Artificiales / Modelos Biológicos Límite: Animals / Humans Idioma: En Revista: Biotechnol Bioeng Año: 2019 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Diferenciación Celular / Técnicas de Cultivo de Célula / Reactores Biológicos / Adipogénesis / Células Madre Mesenquimatosas / Membranas Artificiales / Modelos Biológicos Límite: Animals / Humans Idioma: En Revista: Biotechnol Bioeng Año: 2019 Tipo del documento: Article