A catalytic DNA circuit-programmed and enzyme-powered autonomous DNA machine for nucleic acid detection.

Liu, Shuang; Xin, Chen; Yu, Xiaoxiao; Ding, Zhenbo; Liu, Shufeng

Liu, Shuang; Xin, Chen; Yu, Xiaoxiao; Ding, Zhenbo; Liu, Shufeng.

Afiliación

Liu S; Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, 53 Zhengzhou Road, Qingdao 266042, China. sliu@qust.edu.cn.

Analyst ; 144(20): 5923-5927, 2019 Oct 07.

Article en En | MEDLINE | ID: mdl-31528926

ABSTRACT

ABSTRACT

A catalytic DNA circuit was ingeniously engineered to harness the cleavage of a nicking endonuclease on a triple-stranded duplex DNA probe, autonomously actuating a successive enzyme-powered DNA machine for one-step, isothermal, and autocatalytic nucleic acid analysis. The detection performance could be tuned with or without the use of enzymes.

Asunto(s)

Técnicas Biosensibles/métodos; ADN Catalítico/química; ADN/análisis; Endonucleasas/metabolismo; Técnicas de Amplificación de Ácido Nucleico/métodos; Catálisis; Sondas de ADN; Humanos

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: ADN / Técnicas Biosensibles / ADN Catalítico / Técnicas de Amplificación de Ácido Nucleico / Endonucleasas Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Analyst Año: 2019 Tipo del documento: Article País de afiliación: China

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