Formation lipase cross-linked enzyme aggregates on octyl-modified mesocellular foams with oxidized sodium alginate.
Colloids Surf B Biointerfaces
; 184: 110501, 2019 Dec 01.
Article
en En
| MEDLINE
| ID: mdl-31541891
ABSTRACT
Supported cross-linked enzyme aggregates were prepared by immobilization of Candida antarctica lipase B onto hydrophobic surface of octyl-modified mesocellular foams (MCFs-C8). Oxidized sodium alginate was used as a substitute for traditional glutaraldehyde. Supported cross-linked enzyme aggregates using oxidized sodium alginate (SA-CLEAs@MCFs-C8) exhibited significantly improved thermal stability and organic solvents tolerance compared to the free lipase, lipase adsorbed onto MCFs-C8 and supported cross-linked enzyme aggregates using glutaraldehyde (G-CLEAs@MCFs-C8). Then immobilized lipases were employed for biodiesel production by transesterification of soybean oil with methanol. In the optimization condition, SA-CLEAs@MCFs-C8 were quite stable and still showed high fatty acid methyl esters (FAME) yield after 5 repeated cycles (from 89% to 78%), whereas MCFs-C8-CALB retained 67% FAME yield (about 72% for G-CLEAs@MCFs-C8).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Fúngicas
/
Reactivos de Enlaces Cruzados
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Alginatos
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Estructuras Metalorgánicas
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Lipasa
Idioma:
En
Revista:
Colloids Surf B Biointerfaces
Asunto de la revista:
QUIMICA
Año:
2019
Tipo del documento:
Article