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In vivo localization and postmortem stability of benzo[a]pyrene-DNA adducts.
Poirier, Miriam C; Beland, Frederick A; Divi, Kathyayini V; Damon, Alyssa L; Ali, Mehnaz; Vanlandingham, Michelle M; Churchwell, Mona I; Von Tungeln, Linda S; Dwyer, Jennifer E; Divi, Rao L; Beauchamp, Guy; Martineau, Daniel.
Afiliación
  • Poirier MC; Carcinogen-DNA Interactions Section, Laboratory of Cancer Biology and Genetics, CCR, National Cancer Institute, NIH, Bethesda, Maryland.
  • Beland FA; Division of Biochemical Toxicology, National Center for Toxicological Research, USFDA, Jefferson, Arkansas.
  • Divi KV; Carcinogen-DNA Interactions Section, Laboratory of Cancer Biology and Genetics, CCR, National Cancer Institute, NIH, Bethesda, Maryland.
  • Damon AL; Carcinogen-DNA Interactions Section, Laboratory of Cancer Biology and Genetics, CCR, National Cancer Institute, NIH, Bethesda, Maryland.
  • Ali M; Carcinogen-DNA Interactions Section, Laboratory of Cancer Biology and Genetics, CCR, National Cancer Institute, NIH, Bethesda, Maryland.
  • Vanlandingham MM; Division of Biochemical Toxicology, National Center for Toxicological Research, USFDA, Jefferson, Arkansas.
  • Churchwell MI; Division of Biochemical Toxicology, National Center for Toxicological Research, USFDA, Jefferson, Arkansas.
  • Von Tungeln LS; Division of Biochemical Toxicology, National Center for Toxicological Research, USFDA, Jefferson, Arkansas.
  • Dwyer JE; Carcinogen-DNA Interactions Section, Laboratory of Cancer Biology and Genetics, CCR, National Cancer Institute, NIH, Bethesda, Maryland.
  • Divi RL; Methods and Technologies Branch, Epidemiology and Genomics Research Program, DCPC, National Cancer Institute, NIH, Bethesda, Maryland.
  • Beauchamp G; Département de pathologie et microbiologie, Faculté de médecine vétérinaire, Université de Montréal, St. Hyacinthe, Quebec, Canada.
  • Martineau D; Département de pathologie et microbiologie, Faculté de médecine vétérinaire, Université de Montréal, St. Hyacinthe, Quebec, Canada.
Environ Mol Mutagen ; 61(2): 216-223, 2020 02.
Article en En | MEDLINE | ID: mdl-31569280
DNA adducts of carcinogenic polycyclic aromatic hydrocarbons (PAHs) play a critical role in the etiology of gastrointestinal tract cancers in humans and other species orally exposed to PAHs. Yet, the precise localization of PAH-DNA adducts in the gastrointestinal tract, and the long-term postmortem PAH-DNA adduct stability are unknown. To address these issues, the following experiment was performed. Mice were injected intraperitoneally with the PAH carcinogen benzo[a]pyrene (BP) and euthanized at 24 h. Tissues were harvested either at euthanasia (0 time), or after 4, 8, 12, 24, 48, and 168 hr (7 days) of storage at 4°C. Portions of mouse tissues were formalin-fixed, paraffin-embedded, and immunohistochemically (IHC) evaluated by incubation with r7,t8-dihydroxy-t-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE)-DNA antiserum and H-scoring. The remaining tissues were frozen, and DNA was extracted and assayed for the r7,t8,t9-trihydroxy-c-10-(N 2 -deoxyguanosyl)-7,8,9,10-tetrahydrobenzo[a]pyrene (BPdG) adduct using two quantitative assays, the BPDE-DNA chemiluminescence immunoassay (CIA), and high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ES-MS/MS). By IHC, which required intact nuclei, BPdG adducts were visualized in forestomach basal cells, which included gastric stem cells, for up to 7 days. In proximal small intestine villus epithelium BPdG adducts were visualized for up to 12 hr. By BPDE-DNA CIA and HPLC-ES-MS/MS, both of which used DNA for analysis and correlated well (P= 0.0001), BPdG adducts were unchanged in small intestine, forestomach, and lung stored at 4°C for up to 7 days postmortem. In addition to localization of BPdG adducts, this study reveals the feasibility of examining PAH-DNA adduct formation in wildlife species living in colder climates. Environ. Mol. Mutagen. 61:216-223, 2020. © 2019 Wiley Periodicals, Inc.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Benzo(a)pireno / Carcinógenos Ambientales / Aductos de ADN Límite: Animals Idioma: En Revista: Environ Mol Mutagen Asunto de la revista: BIOLOGIA MOLECULAR / SAUDE AMBIENTAL Año: 2020 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Benzo(a)pireno / Carcinógenos Ambientales / Aductos de ADN Límite: Animals Idioma: En Revista: Environ Mol Mutagen Asunto de la revista: BIOLOGIA MOLECULAR / SAUDE AMBIENTAL Año: 2020 Tipo del documento: Article